Molecular Cloning and Characterization of Bovine CYP26A1 Promoter

Molecular cloning, characterization and association analysis of the promoter region of the bovine CDK6 gene.

Cyclin-dependent kinase 6 (CDK6) is a key element of D-type cyclin holoenzymes. It is involved in the regulation of the G1-phase of the cell cycle and is considered to be an important candidate gene for selection of body measurement traits through marker-assisted selection. We cloned the promoter sequence of this gene in bovines and found it to share high similarity with that of the human CDK6 ...

Molecular cloning and characterization of human homeobox gene Nkx3.1 promoter.

Nkx3.1 is a prostate-specific homeobox gene related strongly to prostate development and prostate cancer. To study its regulation of transcription, 1.06 kb 5' flanking region of Nkx3.1 gene and its 5' deletion mutants (861, 617, 417 and 238 bp) were obtained by PCR and cloned into pGL(3)-basic, a promoter-less luciferase reporter vector, to examine their promoter activities driving the reporter...

Molecular cloning and characterization of murine caspase-12 gene promoter.

The activation of caspase-12 is involved in endoplasmic reticulum-mediated apoptosis. To investigate how caspase-12 is transcriptionally and translationally regulated, we isolated and sequenced the 5'-flanking region of mouse caspase-12 gene by a PCR-mediated chromosome-walking technique, using mouse genomic DNA as a template. Two DNA fragments of 3,221 and 800 bp were isolated and cloned into ...

Molecular cloning and characterization of Izumo1 gene from bovine testis

A well-characterized sperm specific protein of the Member of immunoglobulin superfamily, IZUMO1, has crucial role in fertilization by mediating sperm binding to the egg plasma membrane in the mouse. However little is known about IZUMO1 in bovine. Here, we describe the molecular cloning and expression analysis of bovine IZUMO1 (bIZUMO1). RT-PCR and Western blot analysis of the bovine tissues ind...

Cloning and Characterization of a Promoter